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fatty fatty

I celebrated my one year anniversary today by discovering every single plate and dish of cells I have teeming with contamination.

Also, for some reason when I make homogenates of mouse skin tissue, they give roughly equal protein estimations between genotypes but one is consistently 3-5x more concentrated than the other. This led me to run 4 15-well gels yesterday and 4 10-well gels today with single-microliter changes in sample volume to try to get one pair that has equal actin bands. But since the lipid content is so high (after 3 rounds of spinning and skimming fat off the top and a 100K trip in the ultracentrifuge), 75% of the samples contorted and/or precipitated somewhere in the stacking gel. I just don't get it.

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Comments

silversledge
May. 6th, 2008 02:15 am (UTC)
Ah, that's better.
floyd_mephit
May. 6th, 2008 02:22 pm (UTC)
creepy, but less creepy...

I like screencapture and outright theft for my icon supply.