Also, for some reason when I make homogenates of mouse skin tissue, they give roughly equal protein estimations between genotypes but one is consistently 3-5x more concentrated than the other. This led me to run 4 15-well gels yesterday and 4 10-well gels today with single-microliter changes in sample volume to try to get one pair that has equal actin bands. But since the lipid content is so high (after 3 rounds of spinning and skimming fat off the top and a 100K trip in the ultracentrifuge), 75% of the samples contorted and/or precipitated somewhere in the stacking gel. I just don't get it.
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- Mephitis mephitis, Philosophiæ Doctor (
floyd_mephit) wrote,
Mephitis mephitis, Philosophiæ Doctor
floyd_mephit
fatty fatty
Also, for some reason when I make homogenates of mouse skin tissue, they give roughly equal protein estimations between genotypes but one is consistently 3-5x more concentrated than the other. This led me to run 4 15-well gels yesterday and 4 10-well gels today with single-microliter changes in sample volume to try to get one pair that has equal actin bands. But since the lipid content is so high (after 3 rounds of spinning and skimming fat off the top and a 100K trip in the ultracentrifuge), 75% of the samples contorted and/or precipitated somewhere in the stacking gel. I just don't get it.
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