Also, for some reason when I make homogenates of mouse skin tissue, they give roughly equal protein estimations between genotypes but one is consistently 3-5x more concentrated than the other. This led me to run 4 15-well gels yesterday and 4 10-well gels today with single-microliter changes in sample volume to try to get one pair that has equal actin bands. But since the lipid content is so high (after 3 rounds of spinning and skimming fat off the top and a 100K trip in the ultracentrifuge), 75% of the samples contorted and/or precipitated somewhere in the stacking gel. I just don't get it.
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- Mephitis mephitis, Philosophiæ Doctor (
floyd_mephit) wrote,
Mephitis mephitis, Philosophiæ Doctor
floyd_mephit
fatty fatty
Also, for some reason when I make homogenates of mouse skin tissue, they give roughly equal protein estimations between genotypes but one is consistently 3-5x more concentrated than the other. This led me to run 4 15-well gels yesterday and 4 10-well gels today with single-microliter changes in sample volume to try to get one pair that has equal actin bands. But since the lipid content is so high (after 3 rounds of spinning and skimming fat off the top and a 100K trip in the ultracentrifuge), 75% of the samples contorted and/or precipitated somewhere in the stacking gel. I just don't get it.
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2013
Well it's been awhile since I've posted about anything here, but this year was more relevant than most. So: 2013 review I finally, finally, moved…
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Updates
So, I bought a new car about 2 months ago - a 2012 Mazda 3. I had to get rid of the old truck, it was on its last leg and the about to become illegal…
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(no subject)
My truck of 11+ years is starting to show its age, sadly.. Not too too long ago I started having weird vibrations coming through the steering wheel…
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